Mon copain m a dit comme toi pour les vampires mais j ai pas rigolé car je savais pas alors j avais pas compris! et il m a expliqué ail/vampire et du coup g bien rit avec toi 
ma p'tite Annie prends soin de toi...avec de l'ail tout l'hiver 
Effect on biofilm tolerance to tobramycin.
It has previously been shown that P. aeruginosa biofilm cells are highly tolerant to antibiotic treatment (3). Davies et al. (9) demonstrated that a QS mutant of PAO1 is more susceptible to sodium dodecyl sulfate (SDS) treatment than the wild-type counterpart. Further, Hentzer et al. (25) showed that biofilms treated with a QSI became susceptible to both SDS and tobramycin.
We tested if garlic extract exhibited a similar effect. Two sets of PAO1 biofilms were allowed to form for 3 days in flow chambers in which the medium contained either no garlic or 1% garlic. Following this, each biofilm was challenged with 340 g of tobramycin/ml for 24 h. The effect of the antibiotic treatment was assessed by live/dead staining.
In Fig. 5D, it can be seen that most of the cells in the biofilm treated with both garlic and tobramycin died (Fig. 5D).
In striking contrast, only the cells in the top layer of the biofilm treated with tobramycin alone were killed (Fig. 5B).
Further, Fig. 5C shows that treatment with garlic extract alone had no effect on biofilm viability.
Comparing the untreated biofilm (Fig. 5A) with a garlictreated one (Fig. 5C), a difference in architecture is observed.
The untreated biofilm exhibited the classical mushrooms of a P. aeruginosa biofilm grown in glucose-containing medium. A
flatter, undifferentiated biofilm was formed when the medium contained 1% garlic extract. This effect would be expected by
QSI treatment, since a QS-defective lasI mutant of PAO1 has been found to form flat, undifferentiated biofilms (9).
3) L´etude suivante prouve que l´ajoene, un compose sulphure de l´ail (qui est produit en milieu huileux apres l´ecrasement de la gousse d´ail) est un compose anti Quorum Sensing efficace in vivo contre les biofilmes de Pseudomonas aeruginosa:Garlic extract was administered as treatment for a mouse pulmonary infection model.
Mice were treated with garlic extract or placebo for 7 days, with the initial 2 days being prophylactic before P. aeruginosa was instilled in the left lung of the mice. Bacteriology, mortality, histopathology and cytokine production were used as indicators.
The garlic treatment initially provoked a higher degree of inflammation, and significantly improved clearing of the infecting bacteria.
The results indicate that a QS-inhibitory extract of garlic renders P. aeruginosa sensitive to tobramycin, respiratory burst and phagocytosis by PMNs, as well as leading to an improved outcome of pulmonary infections.
Traduction (sans garantie):Previous in vitro and in vivo studies revealed a significant inhibition of P. aeruginosa QS by crude garlic extract.
Traduction (sans garantie):By bioassay-guided fractionation of garlic extracts, we determined the primary QS inhibitor present in garlic to be ajoene, a sulfur-containing compound with potential as an antipathogenic drug.
Traduction (sans garantie):Furthermore, ajoene treatment of in vitro biofilms demonstrated a clear synergistic, antimicrobial effect with tobramycin on biofilm killing and a cease in lytic necrosis of polymorphonuclear leukocytes. Furthermore, in a mouse model of pulmonary infection, a significant clearing of infecting P. aeruginosa was detected in ajoene-treated mice compared to a nontreated control group.
5) Dans cette etude in vitro et vivo Allicine (un compose sulphureux produit apres l´ecrasement d´une gousse d´ail par la transformation de l´acide amine Alliin sous l´influence de l´enzyme Allinase-> Allicin est un compose tres instable se transformant spontanement a l´air, dans l´eau und des lipides en d´autres composes sulphureux comme le Diallyldisulfide, l´Ajoene) n´agissait pas in vivo contre les biofilmes de la colonie Campylobacter jejuni :Diallyl sulphide destroyed the EPS structure of the C. jejuni biofilm, after which the sessile cells were killed in a similar manner as planktonic cells. Spectroscopic models can predict the survival of sessile cells within biofilms.
Conclusions Diallyl sulphide elicits strong antimicrobial activity against planktonic and sessile C. jejuni and may have applications for reducing the prevalence of this microbe in foods, biofilm reduction and, potentially, as an alternative chemotherapeutic agent for multidrug-resistant bacterial strains.
6) Une etude montrant l´efficacite d´une creme a base d´ail pour inhiber la formation de biofilmes de bacteries pathogenes dans les plaies de brulure cutanee:Based on these results, we performed an in vivo experiment to assess the prevention or reduction of cecal C. jejuni colonization in broiler chickens when allicin was added to drinking water.
We demonstrated that allicin in drinking water did not have a statistically significant effect on cecal C. jejuni colonization in broilers.
It was assumed, based on in vitro experiments, that the activity of allicin was thwarted by the presence of mucin-containing mucus.
Despite promising in vitro results, allicin was not capable of statistically influencing C. jejuni colonization in a broiler flock, although a trend toward lower cecal C. jejuni numbers in allicin-treated broilers was observed.
"Tu aimes mon nouveau parfum, cheri? Chanail..."
Smilax, Polygoni Cuspidati et Andrographis sont egalement recommandees par Buhner.Anti-Spirochete Mixture as published by Dr. S. Dharmananda1.
•Tu Fu Ling (Smilax Glabra)
•Ching-hao or Qing Hao (Herba Artemisiae Annuae)
•Lian Qiao (Fructus Forsythia)
•Hu-chang or Hu Zhang (Rhizoma Polygoni Cuspidati)
• Chuan Xin Lian (Herba Andrographis)
•Ren Dong Teng (Caulis Lonicerae or Honeysuckle Vine)
Code : Tout sélectionner
Smilax Glabra