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 Sujet du message: protocole anti biofilms
MessagePublié: 18 Mars 2014 17:39 
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ah comme j'ai bien fait de prendre le shilajit !!! et les enzymes , la berberine et autre


Citer:
Our sample Lyme Disease protocols integrate the following biofilm protocol items, outlined below, to address pathogenic biofilm:


1. Systemic (proteolytic and mucolytic) enzymes



(degrade sugars, proteins, and mucus-like slime in biofilm). Besides being listed in the protocol charts, more of these enzymes are found in the "Products to Degrade Biofilms" or the "Proteolytic Enzymes" categories. Systemic enzymes enter the circulatory systems and GI tract.


2. Fulvic acid (addresses minerals and metals in biofilm).*


3. Disodium EDTA (chelates minerals in biofilm and calcium shells from nanoparticles). The only EDTA we use is combined in a controlled dose in the products like Interfase Plus. We don't supply high dose single EDTA products, because at high doses it can be dangerous to mobilize a toxic metal load and it can overwhelm you as a result of its incorrect use. Be careful with your use of this, even via a doctor, and always use binders with it.*


4. Lactoferrin (binds to iron, depriving biofilm matrix of this essential mineral). Lactoferrin is often used in biofilm protocols without Lyme Disease* present. Use 100% Lactoferrin (meaning the single supplement). Practitioners suggest buying the 250 mg. capsules, using brands like Symbiotics, Jarrow, or another single high quality lactoferrin supplement.)



START THE DOSE AT 125 MG OR LESS, as severe Herx. can result if you use too high a dose to start. Slowly work up the dose to a manageable one over a period of weeks. (Get some empty capsules and measure out doses.) We can't stress enough the importance of going slowly.



*If you are experiencing acute Lyme Disease, you may want to omit this item since it is derived from milk and it is questionable if some dried dairy products contain Lyme bacteria (which could be cultured to grow.)


5. cis-2-decenoic acid, contained in Royal Jelly or other sources (acts as a dispersant to dissolve protein, nucleic acids, polysaccharides.) Be sure you take a high quality product, as mold has been detected in some products sold on the internet.


6. Targeted and broad spectrum antimicrobials that cross the blood/brain barrier (address pathogens that reside in biofilm).


7.Bile stimulants, resins, charcoal (assist to release and bind neurotoxins that find their way into the biliary tree and GI tract after biofilm breakdown and reduces their massive re-uptake and recirculation back through the body).


8. Buffers and Alkaline pH products, like buffered Vitamin C (buffers stabilize the pH and neutralize the neurotoxic acid load that results from the die off of pathogens and the disease state. Alkaline pH products and foods create a more alkaline interior milieu during healing protocols).


9. Berberine (natural solvent that blocks the ability of biofilms to stick to epithelial tissue and fibronectin in the body, interferes with biofilm formation, inhibits metabolism of bacteria).


10. Curcumin / Turmeric - at higher levels or concentration (supports liver, breaks down mineralizations, assists in musculoskeletal pain support, enhances immunity). Use curcumin products that are made to be "bioavailable" (able to be dispersed in water) because turmeric and curcumin are fat soluable, not water soluable. Try CuraPro and
Theracurmin.


11. Cell Permeability items (allows for the absorption of fat-soluble antimicrobials and other supplements, maintains integrity of cell membranes - like EFAs, MSM, phospholipids, etc.). MSM, for instance, enhances the cell wall's ability to absorb nutrients, but also appears to inhibit the "stickiness" of pathogens to surfaces. ("Fat soluble" means that the product needs fat to be able to be absorbed by the body. Some items easily absorb through water, but others need fats.)



Note: Lipids are part of biofilms - it is still to be proven if dietary lipids or essential fatty acids are recruited to be a part of the biofilm or if they are nutrients for it. (Lipids include dietary fats, like fats in meats and cheese, etc., along with essential fatty acids). Some doctors feel that lipids feed the pathogens and biofilm, while others disagree and recommend liposomal (made with fats) drinks and supplements, along with coconut oil, fish oils, etc.


Our protocols include some essential fatty acids. If you are concerned about this, take MSM or another item instead to increase cellular permeability of fat soluble nutrients.



12. Toxin Binders (bind to neurotoxins and metals to reduce their recirculation).


13. Fiber (sweeps the colon to absorb and bind toxins, inorganic materials, and metals and assist in elimination; supports colonization of healthy microflora in the gut).


14. MSM (natural solvent that at the correct dose interferes with the adhesion of biofilms to surfaces; makes cell membranes more permeable to nutrients.) Build your dose of MSM slowly, as MSM may mobilize metals that needing binding. With higher doses of MSM, be sure to use binders.


15. Herbs with Detergent Action (some herbs have a detergent action to disrupt the outer cell membrane of the spirochete and possibly the biofilm.) Items like Golden Seal (berberine is derived from goldenseal, oregon grape, and other sources), Grapefruit Seed Extract*, balmony, black currant, soapwort, and walnut have a detergent-like action. Other natural detergent chemicals found in plants include: PALMITIC-ACID, PALMITOLEIC-ACID, RICINOLEIC-ACID


(*Commercially made Grapefruit Seed Extracts have been the subject of controversy due to reports that they may contain chemical preservatives and chemicals like benzethonium chloride, benzalkonium chloride, triclosan, and various parabens. Be sure that your GSE is safe if you are taking that product or take a different broad spectrum anti-microbial.)
Our protocols don't include GSE, and you should know that liquid GSE tastes very bitter and detergent like, making it very unpleasant and caustic to swallow, unless the product is encapsulated.)






18. Essential Plant Oils (chemicals in many volatile oils disrupt the biofilm, inhibit the "quorum sensing" ability of the colony, and turn off the biofilm's pump system.) EPOs are also helpful in degrading biofilm. This is why Biocidin Advanced liquid formula is at the foundation of our early protocols, because it includes essential plant oils, but also broad spectrum antimicrobials. Research Study - Biocidin and Biofilm - from Binghamton University researcher Claudia Marques, Ph. D., and the results are phenomenal. Determines Biocidin® is effective against biofilms of Escherichia coli, Pseudomonas aeruginosa, Candida albicans, Klebsiella pneumoniae and Staphylococcus aureus. The study shows 90% - 99% eradication of biofims in 24 hours. Download a copy of the report written by Dr. Marques.



*Special note: If you are using metal "mobilizer" products that have been recommended by your doctor, you must be certain to bind these mobilized metals, because you can create damage by recirculating released metals.





Regarding the use of Xylitol in biofilm protocols - Be careful with oral Xylitol and start the dose very low, as it can cause considerable upset and painful bloating in the stomach and GI tract. Use binders, antimicrobials, and other essential items if you try it. (Topical Xylitol has been proven to be effective in preventing skin wound biofilm, especially in diabetic skin conditions.)





Additions:

1. Herxheimer Support products are recommended if you experience uncomfortable symptoms of die off. Pathogens in the blood, like free floating planktonic cells, will cause more acute, immediate Herx. response, while biofilm-related Herx. may start 5-10 days later (as you degrade more of the biofilm with enzymes).





2. Diet rich in alkaline foods and supplements (prevents an overly acidic milieu, including Bragg's Apple Cider Vinegar, Malic Acid, Buffered C, Alfalfa, green drinks, etc.). When the biofilm is first being addressed, the milieu is acid, and more conducive to eliminating pathogenic biofilm.





3. Anti-Inflammatory items (mitigates the damage to tissues, lowers cytokine storm and Herx. reaction).


4. Immune Support items (modulates immune response and encourage the activity of NK cells).


5. Probiotics / Prebiotics and specialized anti-biofilm supplements targeted at gut biofilm, such as Syntol.


If you have developed IBS, this points to possible pathogenic biofilm communities in the small intestine.


Consider supplementing with probiotics in the
Bacillus family, as these probiotics have been shown to be effective in helping to address Colitis and IBS (see TruFlora product).


http://www.nlm.nih.gov/medlineplus/drug ... l/790.html


http://www.ncbi.nlm.nih.gov/pubmed/1171 ... t=Abstract


http://www.nlm.nih.gov/medlineplus/drug ... /1185.html



Adjust your diet.Reduce processed carbs, potatoes, sweet potatoes, and too much sugar in the diet, even Stevia, as glucose (and mannose) appear to be nutrients for spirochetes as well as biofilm. If you have significant biofilm problems, expect to adhere to an antibiofilm diet for a number of years. To "Starve or Not to Starve" the biofilm of possible nutrients? Here is an interesting update, from the Montana State University Biofilm Research team:



"A biofilm contains a vast array of different metabolic niches which vary in oxygen concentration, nutrient and ionic concentrations as well as concentrations of waste materials.
Cells within the biofilm matrix vary in growth rate from actively growing to essentially dormant.



"Presumably, in every biofilm, there are niches in which
certain cells are metabolically quiescent (quiet, in the resting phase) due to nutrient deprivation. Nutrient limitation, whether it be in a stationary phase culture or in the depths of a biofilm, may increase resistance by reducing metabolic activity.These cells, having fewer metabolic points of attack, are less susceptible to the action of antimetabolites like antibiotics and disinfectants." 1 Montana State University, The Biofilms Hypertextbook



So, should you starve or not starve the biofilm? If you try to starve the biofilm and planktonic (free floating) cells of dietary nutrients, will you theoretically contribute to dormant, resting phases of bacteria located deeper in the biofilm that are more resistant to antibiotics and antimicrobials? Until we know more, most natural practitioners advise limiting sugar intake in its various food forms, as well as suggest other dietary restrictions.





It is thought that free floating, planktonic cells utilize nutrients at a much higher rate than those inside the biofilm. With planktonic cells metabolizing at a high rate, and cells deeper in the biofilm metabolizing at a much lower rate, then



"This reduced metabolic activity may result in these cells ingesting less of an antimicrobial agent than cells with a higher metabolic rate in a planktonic state, resulting in less susceptibility to antimicrobials."


2 http://www.cs.montana.edu/ross/personal ... lms-s4.htm



Remember: If you take mineral supplements like selenium, zinc, and molybdenum (for brain fog), take them hours away from EDTA and Fulvic Acid. Avoid taking calcium, magnesium or iron unless you are in a deficient state. These minerals support the biofilm.

_________________
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mon blog plus de 10 900 articles sur les MVT
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pour trouver quelque chose , taper droopyyoupi + le mot dans google ça va plus vite !

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 Sujet du message: Re: protocole anti biofilms
MessagePublié: 21 Mars 2014 13:23 
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Enregistré le: 20 Mai 2010 10:56
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Bonjour

Votre message contient des liens qui amènent nul part, on ne sait pas d' ou vient ce protocole et on ne comprends rien à rien....


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 Sujet du message: Re: protocole anti biofilms
MessagePublié: 21 Mars 2014 17:07 
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Enregistré le: 25 Août 2010 19:03
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http://flash.lymenet.org/scripts/ultima ... 118508;p=0

suffit de prendre un peu du texte et faire recherche


les liens de pubmed marchent tres bien

il suffit de lire ce qui est encadré c'est tres clair


certains ont été désactivés , ou alors c'est privé


http://www.whyamistillsick.com/


Citer:
Go to the homepage for two Lyme Videos on the right side of the page.

Biofilm Articles and Research

* Understanding Biofilm, by Amy Proal, Bacteriality.com1
* Interview with Dr. Randall Wolcott, bacterial biofilm wound specialist, by Amy Proal, Bacteriality.com
* Biofilm research from Montana State University's Center for Biofilm Engineering
* Click here for a 2010 YouTube video describing biofilms.
* Eye opening article on Biofilms
* Biofilm info from Center for Genome Science ; http://centerforgenomicsciences.org/res ... _large.jpg
* June 2001 - Can Viruses Form Biofilm?
* 2009 Biofilm video explanation: http://www.youtube.com/watch?v=lUcMGktSc7c Featuring Dr. Vincent Fischetti.
* 2010 Video explaining biofilm: http://youtu.be/3eQhNyX7DRQ Featuring Dr. Tim Lu.

* Wound Biofim video: www.youtube.com/user/SWRWoundCareCenter

Bib:
1. Amy Proal's Reference List for Understanding Biofilm Article, above:
REFERENCES

Costerton, J. W., Stewart, P. S., & Greenberg, E. P. (1999). Bacterial biofilms: a common cause of persistent infections. Science (New York, N.Y.), 284(5418), 1318-22. [↩] [↩] [↩] [↩]
Higgins, D. A., Pomianek, M. E., Kraml, C. M., Taylor, R. K., Semmelhack, M. F., & Bassler, B. L. (2007). The major Vibrio cholerae autoinducer and its role in virulence factor production. Nature, 450(7171), 883-6. [↩]
Singh, P. K., Schaefer, A. L., Parsek, M. R., Moninger, T. O., Welsh, M. J., & Greenberg, E. P. (2000). Quorum-sensing signals indicate that cystic fibrosis lungs are infected with bacterial biofilms. Nature, 407(6805), 762-4. [↩] [↩]
Stoodley, P., Purevdorj-Gage, B., & Costerton, J. W. (2005). Clinical significance of seeding dispersal in biofilms: a response. Microbiology, 151(11), 3453. [↩]
O�toole, G. A., & Kolter, R. (1998). Flagellar and Twitching Motility Are Necessary for Pseudomonas Aeruginosa Biofilm Development. Molecular Microbiology, 30(2), 295-304. [↩]
Cho, H., J�nsson, H., Campbell, K., Melke, P., Williams, J. W., Jedynak, B., et al. (2007). Self-Organization in High-Density Bacterial Colonies: Efficient Crowd Control. PLoS Biology, 5(11), e302 EP -. [↩] [↩]
Brockhurst, M. A., Hochberg, M. E., Bell, T., & Buckling, A. (2006). Character displacement promotes cooperation in bacterial biofilms. Current biology: CB, 16(20), 2030-4. [↩]
Parsek, M. R., & Singh, P. K. (2003). Bacterial biofilms: an emerging link to disease pathogenesis. Annual review of microbiology, 57, 677-701. [↩]
Kraigsley, A., Ronney, P., & Finkel, S. Hydrodynamic effects on biofilm formation. Retrieved May 28, 2008. [↩]
Hall-Stoodley, L., Costerton, J. W., & Stoodley, P. (2004). Bacterial biofilms: from the Natural environment to infectious diseases. Nat Rev Micro, 2(2), 95-108. [↩] [↩] [↩]
Lewis, K. (2001). Riddle of biofilm resistance. Antimicrobial agents and chemotherapy, 45(4), 999-1007. [↩] [↩]
Parsek, M. R., & Singh, P. K. (2003). Bacterial biofilms: an emerging link to disease pathogenesis.Annual review of microbiology, 57, 677-701. [↩]
Trampuz, A., Piper, K. E., Jacobson, M. J., Hanssen, A. D., Unni, K. K., Osmon, D. R., et al. (2007). Sonication of Removed Hip and Knee Prostheses for Diagnosis of Infection. N Engl J Med, 357(7), 654-663. [↩]
Ristow, P., Bourhy, P., Kerneis, S., Schmitt, C., Prevost, M., Lilenbaum, W., et al. (2008). Biofilm formation by saprophytic and pathogenic leptospires. Microbiology, 154(5), 1309-1317. [↩]
Moreau-Marquis, S., Stanton, B. A., & O�Toole, G. A. (2008). Pseudomonas aeruginosa biofilm formation in the cystic fibrosis airway. Pulmonary pharmacology & therapeutics. [↩]
Hall-Stoodley, L., Hu, F. Z., Gieseke, A., Nistico, L., Nguyen, D., Hayes, J., et al. (2006). Direct Detection of Bacterial Biofilms on the Middle-Ear Mucosa of Children With Chronic Otitis Media. JAMA, 296(2), 202-211. [↩]
Imamura, Y., Chandra, J., Mukherjee, P. K., Lattif, A. A., Szczotka-Flynn, L. B., Pearlman, E., et al. (2008). Fusarium and Candida albicans Biofilms on Soft Contact Lenses: Model Development, Influence of Lens Type, and Susceptibility to Lens Care Solutions. Antimicrob. Agents Chemother., 52(1), 171-182. [↩]
James, G. A., Swogger, E., Wolcott, R., Pulcini, E. D., Secor, P., Sestrich, J., et al. (2008). Biofilms in Chronic Wounds. Wound Repair and Regeneration,16(1), 37-44. [↩]
Marshall, T. G. (2006b). A New Approach to Treating Intraphagocytic CWD Bacterial Pathogens in Sarcoidosis, CFS, Lyme and other Inflammatory Diseases. [↩] [↩]
Marshall, T. G., & Marshall, F. E. (2004). Sarcoidosis succumbs to antibiotics�implications for autoimmune disease. Autoimmunity reviews, 3(4), 295-300. [↩] [↩]
Sr, G. J. D., & Woody, H. B. (1997). Bacterial persistence and expression of disease. Clinical Microbiology Reviews, 10(2). [↩]
Marshall, T. G. (2007). Bacterial Capnine Blocks Transcription of Human Antimicrobial Peptides. Nature Precedings. [↩]
Morrison, H. I., Ellison, L. F., & Taylor, G. W. (1999). Periodontal disease and risk of fatal coronary heart and cerebrovascular diseases. Journal of cardiovascular risk, 6(1), 7-11. [↩]
Stewart, R., & Hirani, V. (2007). Dental Health and Cognitive Impairment in an English National Survey Population. Journal of the American Geriatrics Society, 55(9), 1410-1414. [↩]
Falkinham Iii, J. O., Iseman, M. D., Haas, P. D., & Soolingen, D. V. (2008). Mycobacterium avium in a shower linked to pulmonary disease. Journal of water and health, 6(2), 209-13. [↩]
Lewis, K. (2001). Riddle of biofilm resistance. Antimicrobial agents and chemotherapy, 45(4), 999-1007. [↩]
Starner, Timothy D et al. 2008. Subinhibitory Concentrations of Azithromycin Decrease Nontypeable Haemophilus influenzae Biofilm Formation and Diminish Established Biofilms. Antimicrobial agents and chemotherapy 52(1):137-45. [↩]
Cogan, N. G., Cortez, R., & Fauci, L. (2005). Modeling physiological resistance in bacterial biofilms. Bulletin of mathematical biology, 67(4), 831-53. [↩]
Marshall, T. G. (2006). VDR Nuclear Receptor Competence is the Key to Recovery from Chronic Inflammatory and Autoimmune Disease. [↩]

_________________
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mon blog plus de 10 900 articles sur les MVT
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 Sujet du message: Re: protocole anti biofilms
MessagePublié: 23 Mars 2014 00:10 
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The Calgary Biofilm Device (CBD) has been described as a technology for the rapid and reproducible assay of biofilm susceptibilities to antibiotics. In this study a simple and inexpensive alternative to the CBD was developed from polypropylene (PP) microcentrifuge tubes and pipette tip boxes. The utility of the device was demonstrated using Candida glabrata, a yeast that can develop antimicrobial-resistant biofilm communities. Biofilms of C. glabrata were formed on the outside surface of microcentrifuge tubes and examined by quantitative analysis and scanning electron microscopy. Growth of three C. glabrata strains, including a clinical isolate, demonstrated that biofilms could be formed on the microcentrifuge tubes. After 24 h incubation the three C. glabrata strains produced biofilms that were recovered into cell suspension and quantified. The method was found to produce uniform and reproducible results with no significant differences between biofilms formed on PP tubes incubated in various compartments of the device. In addition, the difference between maximum and minimum counts for each strain was comparable to those which have been reported for the CBD device.



penser aussi que candida : biofilms


http://www.gestaltreality.com/2013/09/1 ... -biofilms/

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Et si tout le monde adhérait à l'association ET devenait bénévole ce serait bien non ?
http://www.forumlyme.com/phpBB3/viewtopic.php?f=3&t=3325

mon blog plus de 10 900 articles sur les MVT
http://droopyyoupi.blogspot.fr
pour trouver quelque chose , taper droopyyoupi + le mot dans google ça va plus vite !

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 Sujet du message: Re: protocole anti biofilms
MessagePublié: 15 Juin 2014 21:11 
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http://www.ncbi.nlm.nih.gov/pubmed/22862808

The anti-biofilm activity of lemongrass (Cymbopogon flexuosus) and grapefruit (Citrus paradisi) essential oils

on va se mettre aussi à la citronnelle

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Et si tout le monde adhérait à l'association ET devenait bénévole ce serait bien non ?
http://www.forumlyme.com/phpBB3/viewtopic.php?f=3&t=3325

mon blog plus de 10 900 articles sur les MVT
http://droopyyoupi.blogspot.fr
pour trouver quelque chose , taper droopyyoupi + le mot dans google ça va plus vite !

code JFS682 , réduction 5% sur chaque commande http://www.iherb.com
globalmail seulement pour la livraison


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 Sujet du message: Re: protocole anti biofilms
MessagePublié: 16 Sep 2014 17:57 
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Enregistré le: 12 Mai 2013 18:36
Messages: 1672
Plantes anti-biofilme et anti-QS:

*Allium sativum (ail-> principe actif ajoene, l´ail est recommande par le Dr.Zhang, le Dr,Klinghardt):

http://mic.sgmjournals.org/content/151/12/3873.long

http://www.ncbi.nlm.nih.gov/pubmed/19878318

http://www.ncbi.nlm.nih.gov/pubmed/22314537

L´ajoene se forme quand l´ail ecrase entre en contact avec de l´huile.

*Polygonum cuspidatum (Buhner):

http://www.ncbi.nlm.nih.gov/pubmed/17543483

*Andrographis paniculata (Buhner):

http://www.ncbi.nlm.nih.gov/pubmed/22802260

http://www.ncbi.nlm.nih.gov/pubmed/23126203

http://www.ncbi.nlm.nih.gov/pubmed/21422201

Andrographis p. agit en synergie avec des antibiotiques, en particulier avec Azithromycine:

http://www.ncbi.nlm.nih.gov/pmc/article ... figure/F2/

*Houttuynia cordata (Dr.Zhang, Dr.Cowden):

http://www.ncbi.nlm.nih.gov/pubmed/23724685

http://www.ncbi.nlm.nih.gov/pubmed/22996347

http://www.ncbi.nlm.nih.gov/pubmed/23397733

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 Sujet du message: NAC N-Acetylcysteine
MessagePublié: 19 Nov 2014 18:17 
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NAC N-Acetylcysteine

viewtopic.php?f=107&t=5860

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MessagePublié: 30 Déc 2014 12:39 
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Cláudia Marques, PhD
Binghamton University Biological Sciences Dept.

Preliminary Report on Activity of Biocidin against Multiple Species of Biofilms

http://www.biocidin.com/product_images/ ... y-2013.pdf

Citer:
Ingredients:

Bilberry extract (25% anthocyanosides), Noni, Milk Thistle, Echinacea (purpurea & angustifolia), Goldenseal, Shiitake, B. Willow (bark), [b]Garlic
, Grapeseed extract (min 90% polyphenols), Black Walnut (hull and leaf), Raspberry, Fumitory, Gentian, Tea Tree oil, Galbanum oil, Lavender oil (plant and flower), Oregano oil (plant and flower).[/b]


http://www.biocidin.com/biocidin-capsules/

The Gut Connection – !
How Biofilms, LPS and Intestinal Permeability Influence Systemic Inflammation!

!
Dr. Rachel Fresco, L. Ac., Ph. D

http://www.holtorfmed.com/uploads/video ... cp2014.pdf

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Bises et bon retablissement!
Framboise


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